TitleAntimicrobial resistance and co-selection phenomenon in Listeria spp. recovered from food and food production environments.
Publication TypeJournal Article
Year of Publication2013
AuthorsKovacevic, J, Sagert, J, Wozniak, A, Gilmour, MW, Allen, KJ
JournalFood Microbiol
Volume34
Issue2
Pagination319-27
Date Published2013 Jun
ISSN1095-9998
KeywordsAnimals, Anti-Bacterial Agents, Cattle, Dairy Products, Drug Resistance, Multiple, Bacterial, Fishes, Food Contamination, Food Handling, Listeria, Seafood
Abstract

Antimicrobial resistance (AMR), co-selection phenomenon, and the relationship between reduced susceptibility (RSC) to ciprofloxacin (CIP) and resistance to other antimicrobials in Listeria spp. (n = 103) recovered from food processing environments (FPE) and food were investigated. Resistance of Listeria monocytogenes and other listeriae, respectively, to cefoxitin (FOX; 98% vs. 88%), CIP (7% vs. 4%), clindamycin (CLI; 33% vs. 59%) and tetracycline (6% vs. 8%) was observed, as was RSC to CIP (67% vs. 57%) and CLI (65% vs. 41%). L. monocytogenes also possessed RSC to linezolid (LZD; 6%), rifampicin (2%) and streptomycin (6%), with other listeriae displaying RSC to chloramphenicol (4%). L. monocytogenes serotype 1/2a (90%) isolates were more frequently resistant or possessed RSC to CIP compared to serotype 4b (55%) (p = 0.015). When eight strains were experimentally adapted to high concentrations of CIP, co-selection occurred as MICs to benzalkonium chloride (BAC) increased (n = 5), gentamicin MICs remained the same (n = 6) or increased 2-fold (n = 2), and led to RSC to LZD (n = 1) and resistance to CLI (n = 8). Overall, levels of resistance/RSC to CIP in food chain isolates, particularly 1/2a, are concerning. Further, reduced sensitivity to disparate antimicrobials following CIP exposure highlights the need for increased knowledge of co-selection phenomenon linked with antimicrobial agents.

DOI10.1016/j.fm.2013.01.002
Alternate JournalFood Microbiol.
PubMed ID23541198